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For the conjugation of Mn/TI@mHSA to cPLT, Mn/TI@mHSA NPs in PBS were first activated with sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate, then concentrated using Amicon ultra-centrifugal filters (molecular weight cut-off 50 kDa) with 3000 g for 10 min and washed with PBS to remove unreacted linker

Pigmentation lightening for melanin-based dark circles requires 8-12 weeks minimum as brightening peptides and niacinamide gradually inhibit melanin production and promote hyperpigmented cell turnover