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After denaturing in a boiling water bath, the proteins were separated by SDSpolyacrylamide gel electrophoresis (SDSPAGE), transferred to a PVDF membrane, blocked with 5% milk in TBST for 30 min at room temperature, and then incubated with primary antibody at 4 C overnight with shaking

Pointofcare devices have also improved clinic workflow efficiency, enabling wider population screening and realtime risk stratification in primary care settings

In contrast, 2-AR is less abundant and primarily localized to hepatocytes, where it modulates oxidative stress responses 20

This issue converges with another challenge: doses applied in many in vivo studies do not correspond to plastics concentrations found in the environment, and studies using environmentally relevant doses showed no effects, diverging from high doses experiments