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B CELLS The effect of Meth on B cells has been the least studied among all subsets of cells constituting the immune system

B-vitamin assessment Folate and vitamin B 12 status were assessed by measuring serum levels using an electrochemical-immunoluminescence automatized immunoassay system, which was provided by Advia-Centaur (Siemens-Healthcare, Barcelona, Spain)

mansoni TGR was amplified by PCR (forward primer 5-CTATTTGGCTAGACGTCTGT-3 and reverse primer 5-AATACAGTTTCCTTCCCGTT-3) and cloned into PCRII-TOPO vector (Invitrogen, The resulting construct was linearized using XhoI (for SP6 RNA polymerase transcription) and SacI (for T7 RNA polymerase transcription)

For batch fermentation, pre-cultured cells were harvested and inoculated into main cultures to an initial optical density at 600 nm (OD 600 ) of 1.0