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While challenging the importance of the protein binding hypothesis, it was instead suggested that the key mechanism of cell death involves P450-derived ROS formation during APAP metabolism, which triggers extensive LPO [59, 60]

RomualdoGRPrataGBda SilvaTCEvangelistaAFReisRMVinkenMet al

In brief, chemical standard libraries of 149 metabolites in seven pooled libraries were prepared and serially diluted in high-performance liquid chromatography (HPLC)-grade water from in a dilution series from 5 mM to 1 M to generate external standard pools, which are used for calibration of isotopically labelled internal standards and to quantify concentrations of metabolites for which internal standards were not available

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