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Less acid means less B12 gets liberated from food proteins in the first place

A., Morisset, M., Kanny, G., Mgret-Gabeaux, M

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Separation was achieved on a Waters Premier BEH Z-HILIC column (1.7 m, 2.1 150 mm) using 20 mM ammonium bicarbonate solution with 0.1% ammonium hydroxide and 0.1% InfinityLab deactivator as mobile phase A and H 2 O/ACN (1:9 ratio) with 0.1% InfinityLab deactivator as mobile phase B