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For signicant deciencies, injections might be administered more frequently at the start (e.g., weekly), and once levels stabilize, the frequency can be reduced (e.g., monthly)

M., et al., Sex and Gender Differences in the Molecular Etiology of Parkinson's Disease: Considerations for Study Design and Data Analysis, Biology of Sex Differences 16, no

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The elution was concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column that was pre-equilibrated with 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 1 M peptide, 0.01% (w/v) LMNG and 0.0006% (w/v) CHS