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Quantification of Cystatin C immunostaining in skin biopsies Cystatin C immunostaining in the patient biopsies was quantified by semiautomated image analysis using the ImageJ software ( Bright-field images of a section from all individuals were captured on a Nikon Eclipse 50i microscope equipped with a Nikon DS-Fil digital camera and a Nikon Digital Sight DS-U2 camera controller at a resolution of 2560 1920 pixels using a Nikon 4/0.3NA objective

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