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Figure 3 is a schematic of the processes by which impaired copper homeostasis can lead to neurodegenerative disease, and the hypothesis that copper trapping in plaque regions may be protective

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GSH, NAC, trolox, ferrostatin-1, Q-VD, DFO and SAS were from Sigma-Aldrich

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physical methods involving mechanical attrition (e.g., pearlmilling technology, Elan Nanosystems), super critical CO2 and interfacial deposition following solvent displacement

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The EDTA solution had been previously de-aerated with nitrogen gas

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