4.5 Live cell imaging Confocal images of 4 optical planes interspaced by 1 m (from -1.0 m to 2.0 m included) across 25 x 25 fields of view with a 10% overlap were acquired per well on an Opera Phenix High Content Screening System (PerkinElmer) using a 40x or 63x/1.15 NA water-immersion objective, with live cell parameters (37 C and 5% CO 2 )
Subsequently, the released ferrous/iron ions will react with excess hydrogen peroxide in the cell to produce a Fenton-like reaction generating hydroxyl free radicals (OH), and induce ferroptosis of tumor cells [205]
[DOI] [PMC free article] [PubMed] [Google Scholar] 53.Harvey C., Thimmulappa R., Singh A., Blake D., Ling G., Wakabayashi N., Fujii J., Myers A., Biswal S
Connect Tissue Res 24(2):107120 Suguna L, Sivakumar P, Chandrakasan G (1996) Effects of Centella asiatica extract on dermal wound healing in rats