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Further reading [edit] "ACMG Practice Guideline: lack of evidence for MTHFR polymorphism testing"

The absorbance at 546 nm is measured in a Shimadzu UVPC 2100 spectrophotometer (A 546 16 = mg Hb/mL) against a blank containing water instead of hemolysate

Uptake and release in CPM (counts per minute) were normalized to protein quantity as determined by BCA Assay (Thermo Fisher Scientific, 23225)

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