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Glutathione boosts the utilization and recycling of other antioxidants, namely vitamin C, E, alpha lipoic acid and CoQ10. NAC is able to increase cell protection against oxidative stress which is accomplished through the release of cysteine, which is the rate-limiting precursor in the synthesis of glutathione

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To enhance the fluxes to l-cysteine and glycine, we knocked out sdaA to construct the double mutant MG012 by deletion of sdaA in the tnaA mutant MG001