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Remove the vial from the refrigerator and let it sit at room temperature for 15 to 20 minutes before trying again

This pathway activation results in increased endothelial nitric oxide synthase (eNOS) activity, promoting vasodilation and new blood vessel formation

The paper concludes by proposing a unified mechanism whereby BBB dysfunction contributes to neurodegenerative disorders, highlights potential BBB-focused therapeutic strategies and targets, and outlines lessons learned and future research directions