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After 12 h, the medium was replaced, and cells were cultured for another 48 h
However, the interaction between nutrition and fertility appears critical for the reproductive performance and the relationship between ovulatory disorders and metabolic diseases such as diabetes and/or galactosemia suggests that dietary factors exert etiological role in some variants of infertility ( A study performed in 2006 on 12,579 subjects from the Southampton Women's Survey has demonstrated that female and male preconceptional nutritional status influences fertility and perinatal conditions ( This observational prospective study performed on 161 women from the Food, Lifestyle and Fertility Outcome Project has revealed that a diet rich in fish, legumes, vegetables and low in carbohydrates was linearly related with red blood cell folate and vitamin B6 in blood and follicular fluid, with a 40% increase in the chance of pregnancy by IVF intracytoplasmic sperm injection (ICSI) ( A case-control study on 485 women from the Seguimiento Universidad de Navarra (SUN) Project suggested that reproductive outcomes were improved in couples with a correct balanced diet ( The role of different nutrients that could have an impact on female fertility are summarized in the following sections
doi: 10.1007/s00213-010-1870-3
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