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For keeping levels up, shots are usually given monthly

Punwani 2012)

in colon tissue, assessing NO levels and oxidative stress (MDA levels) (as result of the lysis of endothelial cells[9,10]) and ischemia/reperfusion injury, both as a spontaneous course [when blood supply was deprived via ligation (IC rats)] and a more exaggerated course (after ligation removal (IC + RL rats)) in immediate post-ligation time