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Fresh rat blood was obtained prior to each experiment via jugular vein catheters from adult male Sprague-Dawley rats

PARPs consume NAD+ during DNA repair processes, and chronic DNA damage which accumulates with age progressively depletes cellular NAD+ reserves

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Additionally, intracranial (superior sagittal sinus), portal, and caval hypertension and aortal hypotension were reduced, as were the grossly congested stomach and major hemorrhagic lesions, brain swelling, venous and arterial thrombosis, congested inferior caval and superior mesenteric veins, and collapsed azygos vein