After TMB substrate solution is added, only those wells that contain Human ADP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

1.1 Role of complementary and integrative medicine in breast cancer Conventional therapies have improved our understanding of the disease and increased survival rates, but they may not be able to address the complete range of physiological, psychological, or quality-of-life needs that breast cancer patients may have, which is why complementary or integrative medicine (CIM) has emerged as a vital component of overall breast cancer treatment ( Widely studied supplements include curcumin, green tea catechins (EGCG), resveratrol, quercetin, genistein (soy isoflavones), omega-3 fatty acids, silymarin, ashwagandha and vitamins D and selenium as well as some of the mushrooms such as Ganoderma lucidum many of which have preclinical evidence for inhibiting tumour proliferation, chemosensitizing cancer cells modulating oestrogen receptor signalling decreasing oxidative stress or stimulating immune surveillance ( Unlike prior reviews that primarily summarize bioactive compounds, the present manuscript adopts an integrative translational framework that combines molecular mechanisms, clinical evidence, safety evaluation, pharmacokinetic considerations, nano-delivery innovations, and precision oncology perspectives

Some patients report weight loss as early as 2 weeks into the program Clinical effectiveness may vary due to individual factors such as age, weight, diet, exercise, kidney function, and other medical conditions
The physiology is the intervention