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Clinicians use it in the prevention and treatment of macrocytic anemia associated with vitamin B12 deficiency

It has a molecular weight of approximately 403.9 Da in its free peptide form (or ~340.4 Da without the copper ion)

However, due to lifestyle factors such as limited sun exposure and dietary insufficiencies, many individuals experience Vitamin D deficiency, which can manifest as fatigue, depression, and weakened immune response