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To compare the expression of these genes in response to H 2 O 2 , we measured by qPCR the transcriptional level of each one in the pneumococcal R801strain (derivate of the R6 strain) [31] exposed at 20 mM H 2 O 2 for 30 min, and referred to gyrB as internal control [26]

PMC 12263609

Superoxide dismutase assays also demonstrated a statistically significant increase in enzyme activity, suggesting restoration of antioxidant defense after suppression in inflamed colonic tissue, with subsequent improvement in inflammatory abdominal pain in inflammatory bowel disease rats (62)