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The cDNA samples were then prepared for Real-Time Polymerase Chain Reaction (RT-PCR) by using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad Laboratories, Inc, Cat# 172-5271), forward and reverse primers, and cDNA according to the kit protocol

579 In addition, metabolic disorders of neuronal Ca 2+ can drive ferroptosis through interactions with iron and crosstalk between the ER and mitochondria, and maintaining Ca 2+ homeostasis to inhibit ferroptosis may be an innovative target for the treatment of AD

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Over the long term, a compromised microbiome may weaken the integrity of your intestinal wall