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400 l of the reagent mixture (30 ml glacial acetic acid, 20 ml phosphoric acid and 1.25 g ninhydrin) was mixed with 200 l supernatant and heated in sealed test tubes at 100 C for 1 h

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10.31128/AJGP/04-23-6805 26 CuiL.PeriniG.PalmieriV.De SpiritoM.PapiM

Random covalent immobilization methods generally link antibodies to the solid foundation through their own free Nhydroxysuccinimide, cyanogen bromide, and amine groups N,N-carbonyldiimidazole, either tosyl or tresyl chloride Alternatively, an amine groups is able to react with either an aldehyde or a free epoxy group present on the activated support