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The two-pathway strains W303-1b/FG and W303-1b/FP were obtained by the integration of linearized pGAPh-gsh2gsh1 and pGAPg-pro1gshB, respectively

Given that an average rat weighing 200 g drinks approximately 4050 ml/day [120], it would consume 3645 mg of NAC daily, corresponding to 180225 mg/kg/day

They were randomly divided in 6 groups with 10 animals each

Postoperative three-dimensional computed tomography confirmed that the intrathecal catheter was inserted through the L2/3 interlaminar space to the original position at the T8/9 level without any damage to the intrathecal catheter and the LSS was decompressed (Fig