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Briefly, cells cultured on a Transwell membrane were washed with PBS containing 0.2 mM CaCl 2 and 2 mM MgCl 2 (PBS-C/M), followed by fixation with 4% paraformaldehyde (Electron Microscopy Sciences, Fort Washington, PA, USA) in PBS-C/M for 10 min at room temperature

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Multiple lines of evidence indicate that chronic HIV infection leads to neuroinflammation [2], including PET studies utilizing TSPO-labelling radioligands