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Methods Overexpression and purification of recombinant proteins The coding regions of ShD14 (GenBank: KR013120.1), ShHTL1 (GenBank: KR013121.1), ShHTL4 (GenBank: KR013124.1), and ShHTL7 (GenBank: KR013127.1) were amplified by reverse transcription PCR, using the total RNA of S

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With a longer half-life than natural IGF-1, IGF-1 LR3 enhances lean muscle mass growth by stimulating satellite cell activation, promoting hyperplasia (the creation of new muscle fibers), and increasing protein synthesis

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