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Interestingly, higher concentrations such as 1 mM and 2 mM did not yield similar protective effects, indicating a dose-dependent response and identifying 0.1 mM as the optimal concentration for reactive oxygen species mitigation [2]

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184 But, in a preclinical study on the DMBA-induced rat mammary carcinoma, this enhanced effectiveness of methotrexate was far outweighted by the growth-stimulating effect of 3-day pretreatment with combined insulin/glucose infusions