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Med Clin North Am 2009

Fatal hypokalemia may occur when severe megaloblastic anemia is treated intensively with vitamin B12, as a result of increased erythrocyte potassium requirements

Cell viability was measured by assaying mitochondrial activity using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) transformation, as previously described ( Measurement of Intracellular Ca 2+ Concentration Intracellular calcium concentration ([Ca 2+ ] i ) was measured using the fluorescent indicator fura-2

By injecting B12, clients get the most bang for their buck as the vitamin does not have to bypass the digestive tract where much of its effectiveness is reduced