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Never freeze a reconstituted solution

However, the increase in molar ratio is entirely explained by the fact that IGF-I and IGFBP-3 increase with the same number of moles per liter, but with a larger relative increase in IGF-I than IGFBP-3 and with IGF-II molar concentrations being unchanged (112)

Carstens (25) also suggested that the consecutive presence of rare codons is the most important factor affecting the expression of a heterologous protein in E