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The dose of 150 nmol/kg sCT was chosen to compare the effect of sCT and cagrilintide in vivo as this dose of sCT shows the same pharmacokinetic response as the 3 nmol/kg of cagrilintide

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Nasal spray protocol: Dose: 200-500 mcg per application Frequency: 1 to 2 times daily Duration: 4 to 8 weeks Nasal delivery is particularly interesting for researchers focused on neurological applications, as the nasal route provides a potential pathway to bypass the blood-brain barrier through the olfactory and trigeminal nerve pathways

Compared to shCtrl cells, RPL22Flag enrichment to PRKAA1 and PRKAA2 mRNAs was reduced in shGDH1 cells, which could be rescued by exogenous -KG (Fig