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doi: 56 Khoshbaten, M

Basically, cyanocobalamin needs to be broken down to cobalamin and cyanide in order to convert to the active form of B12, and some researchers have concerns about the effects of cyanide accumulation in the body with long-term use of cyanocobalamin but the jurys still out on whether thats a cause for concern

The membranes were blocked using 2% BSA in Tris-buffered saline, 0.05% TWEEN (TBS-T) for 60min and incubated overnight at 4C with the indicated primary antibodies